Coding

Part:BBa_K4410003:Design

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-21)


KanR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 409
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 922
    Illegal SapI.rc site found at 1132


Design Notes

Considering the safety of resistance genes, we added the FRT site in the construction of KanR, which can delete the marker gene before in vivo experiments to improve the biological safety of engineering bacteria.


Source

The source of the part will be its sequence which was retrieved from GenBank .

References